Protective Effect of Benidipine Against the Abnormal Electrical Activity in Single Ventricular Myocytes of the Guinea Pig Under Simulated Ischemic Conditions and Reperfusion.

DOI Web Site Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献2件 参考文献64件
  • Tokuno Tomoaki
    Department of Pharmacology & Therapeutics Faculty of Pharmaceutical Sciences,Nagoya City University,3-1 Tanabedori,Mizuhoku,Nagoya 467-8603,Japan
  • Muraki Katsuhiko
    Department of Chemical Pharmacology,Facultyof Pharmaceutical Sciences,Nagoya City University,3-1 Tanabedori,Mizuhoku,Nagoya 467-8603,Japan
  • Watanabe Minoru
    Department of Chemical Pharmacology,Facultyof Pharmaceutical Sciences,Nagoya City University,3-1 Tanabedori,Mizuhoku,Nagoya 467-8603,Japan
  • Imaizumi Yuji
    Department of Pharmacology & Therapeutics Faculty of Pharmaceutical Sciences,Nagoya City University,3-1 Tanabedori,Mizuhoku,Nagoya 467-8603,Japan

この論文をさがす

抄録

Induction of electrical abnormalities(EAs)under simulated ischemic conditions and after reperfusion was measured from single cardiac myocytes isolated from guinea pig ventricle using whole-cell voltage or current clamp with perforated patch variation.Conditions of simulated ischemia were produced by the exchange of medium from the standard one oxygenated with 95%O2−5%CO2 gas(pH7.4)to the modified one, which contained no glucose, 8mM K+ and 30mM sodium-D, L-lactate and was gassed with 90% argon −10%CO2(pH6.6).Under the simulated ischemia for 20min, EAs such as delayed afterdepolarization, early afterdepolarization, automatic activity or transient inward current were observed in about 37% of myocytes driven electrically at 1Hz.Irreversible hypercontracture occurred in myocytes of 10% or less.Upon reperfusion with the standard solution, EAs and hypercontracture were oberved in about 43% and 22% of cells, respectively.Glibenclamide-sensitive current was detected during ischemia, but tended to be enhanced during reperfusion.Amplitude of Ca2+ current and ATP-sensitive K+ current after reperfusion varied widely with time and from cell to cell.When myocytes were pretreated for 10min with 10nM benidipine, a 1, 4-dihydropyridine derivative Ca2+ blocker, the incidence of EAs and hypercontracture was markedly reduced, suggesting the protective effect of benidipine against cardiac cell injury during ischemia and reperfusion.

収録刊行物

  • Jpn.J.Pharmacol.

    Jpn.J.Pharmacol. 82 (3), 199-209, 2000

    公益社団法人 日本薬理学会

被引用文献 (2)*注記

もっと見る

参考文献 (64)*注記

もっと見る

詳細情報

問題の指摘

ページトップへ