Characteristics of Ca2+ Oscillations in Ileal Longitudinal Muscle Cells of Guinea Pig.
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- Satoh Mitsutoshi
- Department of Chemical Pharmacology,Toho University School of Pharmaceutical Sciences,Miyama 2-2-1,Funabashi,Chiba 274-8510,Japan
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- Takayanagi Issei
- Department of Chemical Pharmacology,Toho University School of Pharmaceutical Sciences,Miyama 2-2-1,Funabashi,Chiba 274-8510,Japan
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- Koike Katsuo
- Department of Chemical Pharmacology,Toho University School of Pharmaceutical Sciences,Miyama 2-2-1,Funabashi,Chiba 274-8510,Japan
Bibliographic Information
- Other Title
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- Characteristics of Ca〔2+〕 Oscillations in Ileal Longitudinal Muscle Cells of Guinea Pig
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Abstract
We studied the mechanisms and characteristics of the spontaneously evoked intracellular Ca2+ changes(Ca2+ oscillations)in ileal longitudinal smooth muscle from guinea pig.Two-dimensional images of Ca2+ oscillations were obtained at 33-ms intervals with a Ca2+-sensitive fluorescence probe, fluo-3 using the intensified CCD camera.Nicardipine(10-7M)significantly decreased the maximum level of fluorescence intensity of the Ca2+ oscillations, inhibited the frequency of the oscillations and tended to decrease the basal level of fluorescence intensity.However, tetrodotoxin(3×10-7M)did not affect these oscillations.Phorbol 12, 13-dibutyrate(10-7M)significantly increased the maximum level of fluorescence intensity and the frequency of Ca2+ oscillations, and it changed them to steady and chronometric Ca2+ oscillations.Cyclopiazonic acid(3×10-5M)also significantly increased the frequency of Ca2+ oscillations.Acetylcholine(10-8M)increased the basal and maximum level of fluorescence intensity and the frequency of Ca2+ oscillations, and accelerated their onset.The increase of basal level of fluorescence intensity was then decreased by cyclopiazonic acid treatment.These results suggest that the augmentation of Ca2+ oscillations is mainly due to the activation of L-type Ca2+ channels, which is modulated by protein kinase C, and that the emptying of intracellular Ca2+ stores may activate the Ca2+ oscillations mediated through the increase of Ca2+ influx in ileal smooth muscle of guinea pig.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 82 (4), 317-325, 2000
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001204287741952
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- NII Article ID
- 10008183898
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DC%2BD3cXisl2gsLo%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 5362788
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- PubMed
- 10875751
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I5362788
- https://api.elsevier.com/content/article/PII:S0021519819306687?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819306687?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/82/4/82_4_317/_pdf
- https://search.jamas.or.jp/link/ui/2000186873
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed