Ca^<2+> Buffering Function of Sarcoplasmic Reticulum in Rat Tail Arteries: Comparison in Normotensive and Spontaneously Hypertensive Rats

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The superficial buffer barrier function of the sarcoplasmic reticulum(SR)during rest and that during stimulation with Bay k 8644, an agonist of L−type Ca<SUP>2+</SUP> channels, were compared in endotheliumdenuded strips of tail arteries from 13−week−old normotensive Wistar−Kyoto rats(WKY)and spontaneously hypertensive rats(SHR), by measuring the effects of cyclopiazonic acid(CPA)and thapsigargin that inhibit SR Ca<SUP>2+</SUP>−ATPase and the effect of ryanodine that depletes SR Ca<SUP>2+</SUP>.The addition of 10μM CPA induced a transient contraction that was not significantly different between WKY and SHR.The CPA−induced contraction was strongly inhibited by 100 nM nifedipine and was abolished by Ca<SUP>2+</SUP>−free solution in both strains.Thapsigargin(100 nM)or ryanodine(10 μM)induced similar, small transient contractions in the two strains.The addition of Bay k 8644(1−100 nM)almost failed to induce a contraction in both WKY and SHR.When the strips were preincubated with 10 μM CPA, 100 nM thapsigargin or 10 μM ryanodine, Bay k 8644 induced similar concentration−dependent contractions in the two strains.The amount of Ca<SUP>2+</SUP> stored in the SR, as estimated from the 20 mM caffeine−induced contraction, was not significantly different between WKY and SHR.Our results suggest that the SR of rat tail arteries can buffer a large amount of Ca<SUP>2+</SUP> that enters the cell during the rest and the Bay k 8644 stimulation, and these functions are not altered in SHR.

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  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 83(4), 335-343, 2000-08-01

    公益社団法人 日本薬理学会

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各種コード

  • NII論文ID(NAID)
    10008184790
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    5501279
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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