Intracellular Ca^<2+> Increase in Neuro-2A Cells and Rat Astrocytes Following Stimulation of Bradykinin B_2 Receptor

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Murine neuroblastoma cell line Neuro−2A cells and rat brain astrocytes showed a dose−dependent increase in intracellular Ca<SUP>2+</SUP> in response to bradykinin, when assessed by a single cell image analyzing system.The Ca<SUP>2+</SUP> increase in Neuro−2A cells by bradykinin was also examined by a suspension fluorescent assay using fura−2 loading.The Ca<SUP>2+</SUP> increase in both cases was suppressed by a bradykinin B<SUB>2</SUB> receptor antagonist, Hoe 140, but not by a B<SUB>1</SUB> receptor antagonist, des−Arg−Hoe 140, suggesting that the effect occurred via specific B<SUB>2</SUB> receptor activation.RT−PCR for bradykinin B<SUB>2</SUB> receptor mRNA showed that both Neuro−2A cells and the astrocytes expressed B<SUB>2</SUB> receptor mRNA.Binding of [<SUP>3</SUP>H]bradykinin to Neuro−2A cells was assessed, and a specific binding constant of 0.75 nM was determined.Furthermore, the increase in [Ca<SUP>2+</SUP>]<SUB>i</SUB> by bradykinin could be caused by a release of Ca<SUP>2+</SUP> from storage sites in the endoplasmic reticulum, since thapsigargin and U−73122 attenuated the effect of bradykinin in Neuro−2A as well as in astrocytes.These results indicate that both astrocytes and neuroblastoma Neuro−2A cells stimulated by bradykinin could express a bradykinin B<SUB>2</SUB> receptor−mediated intracellular Ca<SUP>2+</SUP> increase leading to signal transduction.

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  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 84(2), 140-145, 2000-10-01

    公益社団法人 日本薬理学会

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各種コード

  • NII論文ID(NAID)
    10008185550
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    5549207
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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