Characteristics of Monkey Tryptase Purified From Cheek Pouch Vascular Tissues.

DOI Web Site Web Site Web Site Web Site View 1 Remaining Hide 1 Citations 45 References
  • Sakaguchi Masato
    Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan
  • Takai Shinji
    Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan
  • Jin Denan
    Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan
  • Yamada Mayumi
    Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan
  • Miyazaki Mizuo
    Department of Pharmacology, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan

Search this article

Abstract

Tryptase purified from rat and dog tissues has been reported, although the characteristics of these enzymes are different from human tryptase. For pathophysiological studies of human tryptase, studies on species that have a similar tryptase to humans is needed. In this study, we purified monkey tryptase from cheek pouch vascular tissues using heparin affinity and gel filtration columns. The monkey tryptase, which had a molecular weight of 130 kDa by gel filtration, consisted of a tetramer of 33 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The N-terminal sequence showed high homology with tryptases from other species. The optimum pH and temperature were 7.5—9.0 and 25—40°C, respectively. The enzyme was labile in high-KCl buffer, and the optimum KCl concentration was 0.1 M. The enzyme activity was completely inhibited by diisopropyl phosphorofluoridate and leupeptin but not by soybean trypsin inhibitor and α-antitrypsin. The enzyme hydrolyzed vasoactive intestinal peptide but did not affect angiotensin I, somatostatin and bradykinin. In the present study, we first isolated monkey tryptase from cheek pouch vascular tissues and showed that the characteristics of monkey tryptase are very similar to those of human tryptase.

Journal

Citations (1)*help

See more

References(45)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top