Enhancement of Intracellular Cl^- Concentrations Induced by Extracellular ATP in Guinea Pig Ventricular Muscle

この論文にアクセスする

この論文をさがす

著者

抄録

We investigated effects of extracellular ATP on intracellular chloride activities ([Cl<SUP>-</SUP>]<SUB>i</SUB>) and possible contribution of the Cl<SUP>-</SUP>-HCO<SUB>3</SUB><SUP>-</SUP> exchange to this increase in [Cl<SUP>-</SUP>]<SUB>i</SUB> in isolated guinea pig ventricular muscles. The [Cl<SUP>-</SUP>]<SUB>i</SUB> and intracellular pH (pH<SUB>i</SUB>) were recorded in quiescent ventricular muscles using double-barreled ion-selective microelectrode techniques. MgATP at a concentration higher than 0.1mM, induced an increase in [Cl<SUP>-</SUP>]<SUB>i</SUB>, and this increase in [Cl<SUP>-</SUP>]<SUB>i</SUB> was dependent on the concentration of ATP but not on the concentration of magnesium ions present in the perfusion solution. NaADP, but not NaAMP, at a concentration of 0.5mM induced a similar increase in [Cl<SUP>-</SUP>]<SUB>i</SUB> as that induced by MgATP. However, the NaADP-induced increase in [Cl<SUP>-</SUP>]<SUB>i</SUB> was transient and gradually returned to the control level even though NaADP was continuously present. Furthermore, ATP also triggered a transient acidification of pH<SUB>i</SUB>, and both increases in [Cl<SUP>-</SUP>]<SUB>i</SUB> and intracellular H<SUP>+</SUP> induced by ATP were prevented when preparations were pretreated with stilbene derivatives, SITS and DIDS, or perfused with a Cl<SUP>-</SUP>-free solution. Our findings showed that the increased extracellular ATP concentrations might trigger an increase in [Cl<SUP>-</SUP>]<SUB>i</SUB> in ventricular muscles. In light of previous studies showing that cardiac ischemia induced increases in extracellular nucleotide concentrations and [Cl<SUP>-</SUP>]<SUB>i</SUB> in ventricular muscles, we propose that ischemia-induced accumulation of ATP concentration in the extracellular space may be an important factor to trigger increment of [Cl<SUP>-</SUP>]<SUB>i</SUB> during ischemic conditions.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 84(4), 438-448, 2000-12-01

    公益社団法人 日本薬理学会

参考文献:  49件中 1-49件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008186787
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    5612105
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ