Competence Effect of Basic Fibroblast Growth Factor on Cell Cycle in Subcultured Endothelial Cells of Rat Aorta by Flow Cytometry.
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- Kimura Ikuko
- Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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- Okabe Motonori
- Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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- Ogasawara Masaru
- Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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- Kimura Masayasu
- Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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Abstract
With flow cytometry, we investigated the effect of basic fibroblast growth factor (bFGF)-induced competence in subcultured endothelial cells (EC) (4—9-passage) of rat thoracic aorta. The cell population in each phase of the cell cycle was determined by a double staining technique with fluorescein isothiocyanate-conjugated mouse monoclonal antibody against the proliferation-associated nucleus antigen Ki-67 and propidium iodide for total DNA content. EC were cultured in medium containing 5% fetal bovine serum (FBS) for 6 days. After serum-starvation for 2 days, the treatment with bFGF (3—10 ng/ml) for 12 hr promoted the entry of cells into the G1 phase from the G0 phase concentration-dependently. bFGF (10 ng/ml) increased the cell population in the G1 phase by 50% of the total EC, compared with the control culture without bFGF. A further 12—15-hr culture with 1% FBS after bFGF treatment promoted the entry of the cell into the S phase. Thus flow cytometric analysis demonstrates that bFGF stimulates the entry of EC into the G1 phase from the G0 phase.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 71 (1), 21-27, 1996
The Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282679264599040
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- NII Article ID
- 10008188200
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DyaK28XjtF2msLs%3D
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- ISSN
- 13473506
- 00215198
- http://id.crossref.org/issn/00215198
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- PubMed
- 8791168
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed