Hydrogen Peroxide-Induced Apoptosis and Necrosis in Human Lung Fibroblasts : Protective Roles of Glutathione

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Although reactive oxygen species (ROS)-related cell damage has been implicated in pathogenesis of fibrogenetic pulmonary disorders, features of ROS-mediated cell death in human lung fibroblasts are not completely understood. We therefore examined the effects of hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) on cell growth kinetics in human lung fibroblasts (HFL-1 cells) and tested the roles of antioxidants on the H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death (i.e., necrosis and apoptosis) in HFL-1 cells. We found that the relatively low concentrations of H<SUB>2</SUB>O<SUB>2</SUB> ranging from 10 μM to 100 μM induced predominantly apoptosis, whereas higher concentration of H<SUB>2</SUB>O<SUB>2</SUB> ranging 1 mM - 10 mM induced predominantly necrosis in HFL-1 cells. Extracellular supplementation of glutathione (GSH) in culture media significantly abolished the H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death, whereas GSH-depleted cells by pretreatment with buthionine sulfoxime (BSO) were likely to undergo cell death caused by a lower concentration of H<SUB>2</SUB>O<SUB>2</SUB> than normal HFL-1 cells without BSO treatment. These results indicate that H<SUB>2</SUB>O<SUB>2</SUB> induces both necrosis and apoptosis of human lung fibroblasts at least in part through the action of ROS and that modulation of the ROS production inside and outside of cells may influence the cell survival during oxidative insults.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 79(1), 33-40, 1999-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008194067
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4636892
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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