Maitotoxin-Induced Phosphoinositide Hydrolysis Is Dependent on Extracellular but Not Intracellular Ca2+ in Human Astrocytoma Cells.

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  • Nakahata Norimichi
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Yaginuma Toshiko
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Ohizumi Yasushi
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

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  • Maitotoxin-Induced Phosphoinositide Hydrolysis Is Dependent on Extracellular but Not Intracellular Ca〔2+〕in Human Astrocytoma Cells

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Abstract

Since maitotoxin, a potent marine toxin, is known to cause not only Ca2+ influx but also phosphoinositide hydrolysis, we investigated the Ca2+ dependency of maitotoxin-induced phosphoinositide hydrolysis in 1321N1 human astrocytoma cells. Maitotoxin elicited inositol 1, 4, 5-trisphosphate accumulation in a time-dependent manner. In [3H]inositol-labeled cells, maitotoxin stimulated phosphoinositide hydrolysis in an extracellular Ca2+-dependent manner. Maitotoxin also caused an intracellular Ca2+ elevation, which was abolished by an intracellular Ca2+ chelater BAPTA-AM. Interestingly, maitotoxin still caused phosphoinositide hydrolysis in the BAPTA-AM-treated cells. These results indicate that maitotoxin-induced phosphoinositide hydrolysis is dependent on extracellular but not intracellular Ca2+ in 1321N1 human astrocytoma cells.

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