薬物動態研究におけるラジオルミノグラフィー（ＲＬＧ）　バリデーション，マイクロプレート‐ＲＬＧ，定量全身オートラジオグラフィー

• 馬場 茂雄

  Nemoto Life Science Laboratories

書誌事項

タイトル別名

• Radioluminography(RLG) in the Drug Evaluation Studies: Validation, Microplate-RLG and a Quantitative Whole Body Autoradiography.

抄録

Radioluminography (RLG) has been developed as one of the two-dimensional radiodetection techniques and widely applied to the drug evaluation studies. As the validation items of RLG, 1) the uniformity examination in fl-ray sensitivity, 2) the evaluation of background PSL value (PSL_BG) and monitoring radioactive contamimination of IP, and 3) the accurate expression of radioactivity in Bq instead of PSL were pointed out. The uniformity in β-ray sensitivity was examined by analyzing the IPs exposed to a planar ¹⁴⁷Pm radiation source. A small but definite non-unifomiry in sensitivity was observed, which could be attributed to the mechanics of BAS. New methods for accurately evaluating PSL_BG and monitoring radioactive contamination of IP were proposed. The usefulness of microplate-RLG as off-line counting of HPLC was emphasized. A new radiographic method that images the density distribution of lyophilized animal sections of WBA was described. This technique made it possible to measure the thickness (mg/cm²) of each tissue and correct the self absorption of β-ray, and thereby opened a road to a genuine quantitative WBA.

収録刊行物

• 薬物動態

  薬物動態 14 (1), 42-52, 1999

  日本薬物動態学会

詳細情報

• CRID: 1390282679645328640
• NII論文ID: 10008199168
• NII書誌ID: AN10144117
• DOI: 10.2133/dmpk.14.42
• COI: 1:CAS:528:DyaK1MXjslekt78%3D
• ISSN: 09161139
• Web Site: http://www.jstage.jst.go.jp/article/dmpk1986/14/1/14_1_42/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999181461
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可