Enzymatic Synthesis of Nonreducing Trisaccharide Containing Both Lactose and Trehalose as a Part of the Structure.

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  • Ishii Tetsuya
    Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
  • Tanimoto Morimasa
    Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
  • Itagaki Yasuharu
    Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
  • Honda Yoshihiko
    Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
  • Win Than Than
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • Kawase Chihiro
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • Kita Ayano
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • Onodera Syuichi
    Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University
  • Ito Hiroyuki
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • Matsui Hirokazu
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • Honma Mamoru
    Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

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Other Title
  • 乳糖とトレハロースの構造を併せもつ非還元三糖の酵素合成

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Abstract

To synthesize nonreducing oligosaccharide derived from trehalose, enzymatic transgalactosylation was investigated using lactose and trehalose as substrates and a commercially available β-ga-lactosidase, which has a tendency to transfer the galactosyl residue to the C4 position of acceptor sugars, from Bacillus circulans. The galactosylation successfully resulted in the efficient production of O-β-D-galactopyranosyl-(1-4)-α, α-trehalose. This trisaccharide showed good stability against acidic treatment.

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