硫黄酸化細菌の細胞下におけるsulfitedehy drogenase触媒反応の酵素学的解析

• 松本 隆志

  株式会社ミツカングループ本社 京都府立大学農学部生物資源化学科

• 松本 健司

  株式会社ミツカングループ本社

• 大西 正健

  株式会社ミツカングループ本社

書誌事項

タイトル別名

• Kinetic Study of the Sulfite Dehydrogenase-catalyzed Reaction in the Subcellular State of Thiobacillus thiooxidans.

抄録

Based on kinetic studies of the sulfite dehydrogenase-catalyzed reaction investigated using the subcellular fraction instead of the microbe Thiobacillus thiooxidans, it was confirmed that the initial velocity is very proportional on the concentration of the subcellular fraction and the optimal pH is 7.5, which is the same as that obtained in the microbe. The kinetic parameters, Michaelis constant (Km) and maximum velocity (V), were evaluated to be 1.7±0.2 mM and 0.083±0.035 mM/min/mg protein, respectively. The ferricyanide concentration examined was found to be proportional to the initial velocity within 1.5 mM. It was also found that cytochrome c can be absorbed as a second substrate in place of ferricyanide. Thus, a new device should be very possible using the subcellular fraction of T. thiooxidans as a highly sensitive and automatic sensor apparatus for sulfite etermination.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 48 (1), 11-17, 2001

  日本応用糖質科学会

詳細情報

• CRID: 1390282681268927104
• NII論文ID: 10008252277
• NII書誌ID: AN10453916
• DOI: 10.5458/jag.48.11
• ISSN: 18807291
• ISSN: 13447882
• NDL書誌ID: 5648073
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5648073
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/48/1/48_1_11/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可