バナナ果肉の可溶性および細胞壁結合型酸性α-グルコシダーゼの精製と性質
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- 小西 洋太郎
- 大阪市立大学生活科学部食品栄養科学科
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- 原田 美亜
- 大阪市立大学生活科学部食品栄養科学科
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- 中筋 美恵
- 大阪市立大学生活科学部食品栄養科学科
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- D'Innocenzo Marisa
- Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo
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- Lajolo Franco M.
- Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo
書誌事項
- タイトル別名
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- Purification and Characterization of Soluble and Cell Wall-bound Acid .ALPHA.-Glucosidases of Ripe Yellow Banana Pulp.
- Purification and Characterization of Soluble and Cell Wall-bound Acid α-Glucosidases of Ripe Yellow Banana Pulp
- Purification and Characterization of Soluble and Cell Wall bound Acid アルファ Glucosidases of Ripe Yellow Banana Pulp
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抄録
Banana pulp, irrespective of the degree of ripening, contained soluble and cell wall-bound forms of acid α-glucosidases (SAAG and BAAG). BAAG was released with a neutral buffer containing 0.2-2 M NaCI after extracting of SAAG with a salt-free buffer. From the same bunch of ripe yellow bananas, SAAG and BAAG were purified at 732-fold and 264-fold, respectively, using ConASepharose and Sephadex G-150 gel column chromatographies. The molecular weights of SAAG and BAAG were 70, 000 and 90, 000, respectively, by gel filtration. These enzymes were typical maltases that required maltose (G2) and malto-oligosaccharides (G3-G7) as substrates, but not isomaltose, treharose, sucrose, pullulan, glycogen, or soluble starch. The Vmax/Km ratios (apparent hydrolytic efficiencies) of SAAG and BAAG toward G5-G7 were 89-112 and 14-52%, respectively, with the ratio toward maltose being 100%. As for ripe yellow banana where cell wall or amy loplastic membrane has not been maintained, it is speculated that BAAG could access starch and its hydrolytic products and would share in starch degradation in collaboration with amylases and SAAG.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 48 (1), 19-25, 2001
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681268932096
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- NII論文ID
- 10008252290
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- NII書誌ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
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- NDL書誌ID
- 5648087
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可