The Activity, Physiological Role and Biotechnological Application of Potato D-enzyme.

DOI Web Site Web Site 40 References

Bibliographic Information

Other Title
  • 馬鈴薯D-酵素の機能と澱粉加工への利用
  • 平成12年度日本応用糖質科学会奨励賞受賞講演 馬鈴薯D-酵素の機能と澱粉加工への利用
  • ヘイセイ 12ネンド ニホン オウヨウ トウシツ カガクカイ ショウレイショウ ジュショウ コウエン バレイショ D コウソ ノ キノウ ト デンプン カコウ エ ノ リヨウ

Search this article

Abstract

D-enzyme (EC 2.4.1.25) is believed to be involved in starch metabolism, but the function in vivo is not known. In order to investigate the role of D-enzyme, several approaches have been under taken. Transgenic potato plants with dramatically reduced D-enzyme activity were obtained by introducing sense and antisense D-enzyme cDNA sequences with the appropriate promoter sequence. The tubers from these plants sprouted later and the growth of sprouts was slower than the wild type. However, no significant difference was found in starch produced in tubers, either in its quantity or quality. These results seems to suggest the role of D-enzyme in starch degradation rather than starch synthesis. The analysis of the action of D-enzyme on amylose and amylopectin revealed that the enzyme can catalyze the cyclization reaction and produce cycloamylose with DP larger than 17. Cycloamylose produced are highly soluble in water and can incorporate various compounds in their helical cavities, thus expected to find exploitation in food and pharmaceutical industries.

Journal

References(40)*help

See more

Details 詳細情報について

Report a problem

Back to top