Mechanism of Synthesis of Levan by Bacillus natto Levansucrase (糖質関連酵素化学シンポジウム) Mechanism of Synthesis of Levan by Bacillus natto Levansucrase

この論文にアクセスする

この論文をさがす

著者

抄録

Levansucrase acts on sucrose usually to synthesize a fructan which contains a sucrose unit at one terminus of the molecule and has branches formed by β-2, 1-fructofuranosyl linkage. The transfer of fructosyl residue to an acceptor by the enzyme is carried out from not only sucrose but also oli gosaccharides which contain a sucrose unit such as raffinose or fructosyl maltoside, fructosyl cellobioside, fructosyl lactoside, etc., which can be synthesized from sucrose in the presence of an acceptor sugar (maltose, cellobiose, lactose, etc.) by levansucrase. Soybean contains such oligosaccharides other than sucrose. We isolated levan from fermented soybean (Natto) and analyzed the molecular weight and terminus of the molecule. It was revealed that the molecular weight was about 1.1 × 10<SUP>4</SUP> by HPLC using molecular markers of pullulans, and the levan isolated contained a stachyose unit at the terminal of the molecule. It was thought that the enzyme might have a donor binding site (with a high affinity toward sucrose) and an acceptor binding site in the molecule. Because synthesized trisaccharide contained only raffinose and the terminal unit of levan was raffinose in the case of levan synthesized from sucrose in the presence of melibiose, although the levan synthesized from sucrose alone contained kestose (mainly the 1-kestose and 6-kestose, ca .1:1) as the terminal unit of the levan.

収録刊行物

  • 応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience

    応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience 49(2), 229-237, 2002-04-01

    The Japanese Society of Applied Glycoscience

参考文献:  11件中 1-11件 を表示

各種コード

  • NII論文ID(NAID)
    10008253813
  • NII書誌ID(NCID)
    AN10453916
  • 本文言語コード
    ENG
  • 資料種別
    REV
  • ISSN
    13403494
  • NDL 記事登録ID
    6142529
  • NDL 雑誌分類
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL 請求記号
    Z17-15
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ