脂質修飾法を用いる糖加水分解酵素の改変 : 有機溶媒中での配糖化反応 A Facile Transglycosylation Catalyzed by Lipid-coated Glycoside Hydrolases

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抄録

Various lipid-coated glycoside hydrolases were prepared, which were soluble in most organic solvents such as isopropyl ether, isooctane and benzene but insoluble in aqueous solution. They could act as efficient catalysts for transglycosylation of α-D-or β-D-mannoside, N-acetyl-β-D-glucosaminide, β-D-glucoside or β-D-galactoside to hydrophobic acceptor alcohols in dry isopropyl ether. When a native β-D-galactosidase was used in the aqueous solution containing acetonitrile for the same reaction (a conventional method), the yield of the transgalactosylation was low due to the predominant process of hydrolysis reaction. The enzyme activity for glycosylation depends on the coating of lipids as well as the orgin or type of enzyme. These lipid-coated glycoside hydrolases could also act as an efficient catalyst for transglycosylation in the two water-organic phases: both the hydrophobic lipid-coated enzyme and alcohols were solubilized in isopropyl ether and mixed with an aqueous solution of glycosyl donors such as lactose and cellobiose.

収録刊行物

  • 応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience

    応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience 44(3), 337-342, 1997-08-31

    The Japanese Society of Applied Glycoscience

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各種コード

  • NII論文ID(NAID)
    10008257276
  • NII書誌ID(NCID)
    AN10453916
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    13403494
  • NDL 記事登録ID
    4304127
  • NDL 雑誌分類
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL 請求記号
    Z17-15
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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