Brevibacterium sp. No. 9605株の生産するサイクロデキストリングルカノトランスフェラーゼに関する研究 Studies on Cyclodextrin Glucanotransferase from Brevibacterium sp. No. 9605

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A strain of Brevibacterium sp. No. 9605 was isolated from soil and produced cyclodextrin glucanotransferase (CGTase, EC. 2.4.1.19). The CGTase produced mainly γ-cyclodextrin (γ-CD) from sarch in the initial stage of reaction, but then, the proportion of β-CD was increased and also produced a small amount of α-CD. The CDs formation was affected by the presence of calcium ion and ethanol . In the presence of ethanol, the yield of γ-CD from soluble starch was increased . It was thought to be due to the repression of β-CD production from starch and the repression of γ-CD degradation by coupling reaction. Brevibacterium CGTase showed a broad acceptor specificity to various monosaccha rides and phenolic compounds and produced more transfer products of D-mannose, L-rhamnose, 1, 3-dihydroxybenzene, 1, 3, 5-trihydroxybenzene, 3-hydroxybenzyl alcohol, and (+) -catechin using as acceptors than Bacillus macerans and Bacillus stearothermophilus CGTases did. The transfer products of D-mannose and kojic acid were 4-O-α-D-glucosyl-D-mannose and kojic acid 7-O-α-D-glucoside, respectively.

収録刊行物

  • 応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience

    応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience 46(1), 87-95, 1999-03-31

    The Japanese Society of Applied Glycoscience

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各種コード

  • NII論文ID(NAID)
    10008258742
  • NII書誌ID(NCID)
    AN10453916
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    13403494
  • NDL 記事登録ID
    4785651
  • NDL 雑誌分類
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL 請求記号
    Z17-15
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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