Differences in Sugar Regulation between Two Isoforms of .ALPHA.-Amylase in Suspension-Cultured Cells of Rice.

• Geshi Naomi

  Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University

• Mitsui Toshiaki

  Department of Applied Biochemistry, Faculty of Agriculture, Niigata University

• Akazawa Takashi

  Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University

• Yamaguchi Junji

  Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University

Bibliographic Information

Other Title

• イネ培養細胞における2種類のα-アミラーゼアイソフォームの糖による発現制御の差異
• Differences in Sugar Regulation between Two Isoforms of α-Amylase in Suspension-Cultured Cells of Rice
• Differences in Sugar Regulation between Two Isoforms of アルファーAmylase in Suspension-Cultured Cells of Rice

Abstract

Tissue-specific expression of the isoforms of rice α-amylase and their expression in suspension cultured cells were investigated, together with regulation by various sugars. Six isoforms, Y, A, G, H, I and J, were identified: Isof orm A, Isof orm Y and Isof orms A-J were mainly detected in germinating seeds, young plant tissues, and suspension-cultured cells, respectively. From the results of amino acid sequencing of the purified isoforms, isoform A and isoform H were identified as the gene products of RAmy1A and RAmy3D, respectively (Plant Physiol., 110, 1395-1404 (1996)) . Since metabolic regulation of the expression of rice a-amylase has been reported, we analyzed this phenomenon by following the changes in levels of each isoform in cells in suspension cultures in the presence of various sugars . The expression of the RAmy3D-encoded protein (isoform H) was completely repressed by so-called metabolic sugars, while expression of the RAmy1A-encoded protein (isoform A) was repressed to a less extent. The results indicate that high expression of the RAmy3D-encoded protein is controlled much differently from that of the RAmy1A-encoded protein.

Journal

• Journal of Applied Glycoscience

  Journal of Applied Glycoscience 46 (2), 111-119, 1999

  The Japanese Society of Applied Glycoscience

Details

• CRID: 1390282681270487168
• NII Article ID: 10008258769
• NII Book ID: AN10453916
• DOI: 10.5458/jag.46.111
• ISSN: 18807291
• ISSN: 13447882
• NDL BIB ID: 4785681
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4785681
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/46/2/46_2_111/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed