HPAEC-PAD法によるヒアルロン酸オリゴ糖の分離と定量 Separation and Quantitative Analysis of Hyaluronan Oligosaccharides by HPAEC-PAD

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抄録

Hyaluronan oligosaccharides were analyzed using high-performance anion-exchange chromatogra-phy with pulsed amperometric detection (HPAEC-PAD) . The procedure invalves the separation of hyaluronan oligosaccharides on an anion-exchange column, CarboPac PA-1, using alkaline eluants. A gradient is made using these two solutions; solution A (100 mM sodium hydroxide) and solution B (100 mM sodium hydroxide/1.5 M sodium acetate), eluted at 1 mL/min. The unit resolution of hyaluronan oligomers could be achieved up to at least 20 disaccharide units through HPAEC-PAD. Furthermore, integrated pulsed amperometry detected only -40 pmol of hyaluronan oligosaccharide. This analysis system was easily used for the separation and quantitative analysis of hyaluronan oligosaccharides, and applied to the determination of the time course of hyaluronidase digestion of hyaluronan.

収録刊行物

  • 応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience

    応用糖質科学 : oyo toshitsu kagaku = Journal of applied glycoscience 46(3), 299-302, 1999-08-31

    日本応用糖質科学会

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各種コード

  • NII論文ID(NAID)
    10008259295
  • NII書誌ID(NCID)
    AN10453916
  • 本文言語コード
    JPN
  • 資料種別
    NOT
  • ISSN
    13403494
  • NDL 記事登録ID
    4846284
  • NDL 雑誌分類
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL 請求記号
    Z17-15
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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