Preparation and Antioxidative Activity of 1,5-Anhydrofructose.
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- Fujisue Mami
- Nihon Denpun Kogyo Co
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- Yoshinaga Kazuhiro
- Nihon Denpun Kogyo Co
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- Muroya Kenko
- Nihon Denpun Kogyo Co
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- Abel Jun-ichi
- Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
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- Hizukuri Susumu
- Kagoshima University
Bibliographic Information
- Other Title
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- 1,5-アンヒドロフルクトースの調製と抗酸化活性
- Preparation and Antioxidative Activity of l,5-Anhydrofructose
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Abstract
A simple enzymic method for the preparation of 1, 5-anhydrofructose (1, 5-AF) and its antioxidative activity were investigated. α-1, 4-glucan lyase (EC 4 .2.2.13, GLase) was extracted and purified from red seaweed, Gracilaria verrrucosa. One-hundred-and-fifty grams of 1, 5-AF was obtained from 360 g of waxy maize starch by digestion with the purified GLase and the following purification of the product by ion-exchange resins and gel filtration. The purity of the product was 98 .6% by HPLC using an ionexchange resin (MC1 GEL CK08S). The product, 1, 5-AF, was identified by IH-13C COSY NMR, and the [α] D25 was -16.8°. The antioxidative activity of 1, 5-AF was examined by comparison with that of ascorbic acid (VC) for preventing the oxidation of linoleic acid . The antioxidative activity of 1, 5-AF was found to be approximately 1.8-fold that of VC by the methods using thiocyanate and 2-thiobarbituric acid.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (4), 439-444, 1999
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206292886016
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- NII Article ID
- 10008259440
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- NII Book ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
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- COI
- 1:CAS:528:DC%2BD3cXhtFCnsb4%3D
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- NDL BIB ID
- 4976626
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed