16S rRNA Gene Sequence Analysis of Streptomyces lavendulae and Streptomyces virginiae Strains.

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  • 16S r RNA gene sequence analysis of Str

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Abstract

The 16S rRNA gene sequences of 18 strains of Streptomyces lavendulae and Streptomyces virginiae were determined. Fifteen strains including the type strains of S. lavendulae and S. virginiae had 1,514 nucleotides in positions 10 to 1,541 of the Escherichia coli numbering system, and their similarities were more than 98.1%. On the other hand the sequences of S. lavendulae subsp. lavendulae IFO 3361, S. lavendulae subsp. lavendulae IFO 12341 and S. virginiae ATCC 13013 consisted of 1,516 nucleotides. The differences in the number of nucleotides were observed between 3 strains containing 1,516 nucleotides and 15 strains containing 1,514 nucleotides in positions 181 to 219,1,133 to 1,141 and 1,446 to 1,456 (E. coli numbering system) and various sequence lengths of these areas were corresponded to the similarity level of the 16S rRNA gene sequence. The homology values of DNA-DNA hybridization between these three strains and their type strains were very low (15 to 22%) and these three strains were located in a cluster far from the other strains in the cluster analysis based on the taxonomic characteristics (Anzai, Y.; T. Okuda & J. Watanabe, J. Antibiot. 47: 183-193,1994). Therefore, we conclude that S. lavendulae subsp. lavendulae IFO 3361 and IFO 12341, and S. virginiae ATCC 13013 should not be assigned to S. lavendulae or S. virginiae.

Journal

  • Actinomycetologica

    Actinomycetologica 11 (1), 15-19, 1997

    The Society for Actinomycetes Japan

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