Development of a PCR/Southern dot blot based detection system for the presence of genes involved in beta-lactam biosynthesis

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Screening of actinomycetes and fungi for genes involved in beta-lactam production can be carried out using a technique involving both PCR with conserved primers to the isopenicillin N synthase gene and a Southern dot blotting. This technique was successful with nine out of nine known beta-lactam producing Actinomycetes tested and three out of six known beta-lactam producing Fungi tested. It proved unable to detect the isopenicillin N synthase gene in the two <I>Flexibacter</I> beta lactam producing eubacteria strains tested. It also identified the presence of potential beta-lactam production genes in two known none beta-lactam producing actinomycetes and one out of fourteen novel newly isolated actinomycetes. This technique has potential for use in commercial screens for novel beta-lactam producers.

収録刊行物

  • 日本放線菌学会誌

    日本放線菌学会誌 12(1), 29-36, 1998-06-30

    The Society for Actinomycetes Japan

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各種コード

  • NII論文ID(NAID)
    10008262828
  • NII書誌ID(NCID)
    AN10080091
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09145818
  • NDL 記事登録ID
    4524024
  • NDL 雑誌分類
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL 請求記号
    Z18-1070
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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