Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method

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Protoplast fusion between different species of <i>Streptomyces</i> was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants. Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between <i>S</i>. <i>griseus</i> and <i>S</i>. <i>durhamensis</i>, and between <i>S</i>. <i>californicus</i> and <i>S</i>. <i>catenulae</i>, eight and two recombinants, respectively, were obtained using the liquid regeneration method. Conversely, in the case of crosses between <i>S</i>. <i>ornatus</i> and <i>S</i>. <i>catenulae</i>, and between <i>S</i>. <i>ornatus</i> and <i>S</i>. <i>vendargensis</i>, seven recombinants each were obtained using only the agar method. The physiological characteristics, such as the assimilation of carbohydrate and antibiotic resistance, of these fusants differed considerably from those of their parental strains. Using the proposed liquid regeneration method, a simpler and quicker procedure for protoplast fusion is described.

収録刊行物

  • Fisheries science : FS

    Fisheries science : FS 68(2), 395-402, 2002-04

    The Japanese Society of Fisheries Science

参考文献:  10件中 1-10件 を表示

被引用文献:  1件中 1-1件 を表示

  • 海洋細菌と大腸菌の細胞融合および得られた融合株の性状(短報)

    張 恩実 , 今田 千秋 , 鎌田 正純 , 小林 武志 , 濱田(佐藤) 奈保子 , ENSHI ZHANG , CHIAKI IMADA , MASAZUMI KAMATA , TAKESHI KOBAYASHI , NAOKO HAMADA-SATO , 東京海洋大学大学院海洋科学技術研究科 , 東京海洋大学大学院海洋科学技術研究科 , 山野美容芸術短期大学美容芸術学科 , 東京海洋大学大学院海洋科学技術研究科 , 東京海洋大学大学院海洋科学技術研究科 , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Science of Aesthetics and Art Yamano College of Aesthetics , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology

    日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries 72(4), 743-745, 2006-07-15

    CiNii 外部リンク 日本農学文献記事索引 J-STAGE 参考文献16件 被引用文献2件

各種コード

  • NII論文ID(NAID)
    10008273357
  • NII書誌ID(NCID)
    AA10993718
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09199268
  • NDL 記事登録ID
    6152088
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z54-H592
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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