Complete nucleotide sequence of a cDNA encoding a myosin heavy chain from mantle tissue of scallop Patinopecten yessoensis.

• HASEGAWA YASUSHI

  Department of Applied Chemistry, Muroran Institute of Technology

Abstract

It has been reported that the amino acid sequences of striated and catch muscle myosin heavy chains from two scallop species (Argopecten irradians and Placopecten magellanicus) are almost identical, but that the ATPase activities between these myosins vary several-fold. These myosin sequences have been useful for identifying the region that modulates the ATPase activity of scallop myosin. In the present study, a cDNA encoding a myosin heavy chain was isolated from the mantle tissue of scallop Patinopecten yessoensis. The cDNA is composed of 6067 base pairs (bp) including an open-reading frame of 5841 p, which encodes an amino acid sequence of 1947 residues. The deduced amino acid sequence of P. yessoensis mantle myosin had a high identity of 90%, 92%, and 91% to P. magellanicus, A. irradians, and Pecten maximus striated muscle myosins, respectively. Interestingly, while the deduced amino acid sequences of around adenosine triphosphate-binding and actin-binding sites of the mantle myosin are homologous to those of A. irradians striated muscle myosin, the subfragment 2 hinge region and the non-helical tail region are similar to those of catch muscle myosin.

Journal

• Fisheries science

  Fisheries science 68 (2), 403-415, 2002

  The Japanese Society of Fisheries Science

Keywords

• cDNA cloning
• myosin
• scallop

Details

• CRID: 1390282679404881792
• NII Article ID: 130003742442
• NII Article ID: 10008273368
• NII Book ID: AA10993718
• DOI: 10.1046/j.1444-2906.2002.00439.x
• ISSN: 14442906
• ISSN: 09199268
• NDL BIB ID: 6152379
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6152379
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed