Identification of Meat Species by HPLC Patterns of Sarcoplasmic Protein Extracted from Acetone-dried Muscle Powder of Cattle, Horse, Pig, Goat and Sheep.

• MATSUOKA Akiyoshi

  Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture

• FURUKAWA Noboru

  Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture

• TAKAHASHI Tsuyoshi

  Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture

• YAMANAKA Yoshitada

  Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture

Bibliographic Information

Other Title

• 家畜肉から抽出した筋しょうタンパク質のＨＰＬＣ分画パターンによる肉種の判別
• 家畜肉から抽出した筋漿タンパク質のHPLC分画パターンによる肉種の判別
• カチク ニク カラ チュウシュツシタ キンショウ タンパクシツ ノ HPLC

Abstract

The possibility of identification of meat species by HPLC patterns of sarcoplasmic protein was investigated. In this study, sarcoplasmic proteins extracted from M. longissimus thoracis of cattle, horse, pig, goat and sheep which were treated with acetone cooled below -10°C (acetone-dried muscle powder) in order to eliminate the muscle lipid were analyzed by reverse-phase HPLC. The HPLC patterns of the sarcoplasmic protein obtained were then statistically analyzed by discriminant analysis.<br>The reliability of RRt was improved significantly by using sarcoplasmic proteins extracted from acetone-dried muscle powder. There was a tendency that peak area of the protein fractions was different between individuals in a species.<br>The chromatograms of the sarcoplasmic proteins from cattle, horse, pig, goat and sheep revealed the patterns specific to the species, respectively, and suggested that the five species can be distinguished one from another.<br>Results of discriminant analysis showed that the classification rule identified 12 in 13 samples for cattle (92.3%), 16 in 17 samples for horse (94.1%), 25 in 25 samples for pig (100%), 8 in 9 samples for goat (88.9%), and 10 in 10 samples for sheep (100%).

Journal

• Nihon Yoton Gakkaishi

  Nihon Yoton Gakkaishi 34 (4), 163-169, 1997

  The Japanese Society of Swine Science

Keywords

• reverse-phase HPLC
• sarcoplasmic protein
• meat species
• acetone powder
• discriminant analysis

Details

• CRID: 1390001204707958400
• NII Article ID: 10008284843
• NII Book ID: AN10202971
• DOI: 10.5938/youton.34.163
• ISSN: 1881655X
• ISSN: 0913882X
• NDL BIB ID: 4381813
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010570241
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4381813
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed