Effect of Seminal Plasma on the Motility of Boar Spermatozoa Stored at 10.DEG.C. for 28 Days.

• TAUCHI Shizuka

  Faculty of Agriculture, Iwate University

• SAKAI Mikiko

  Faculty of Agriculture, Iwate University

• YAMAUCHI Nobuhiko

  Tohoku National Agricultural Experiment Station

• GESHI Masaya

  Tohoku National Agricultural Experiment Station

• NAGAI Takashi

  Tohoku National Agricultural Experiment Station

• HASHIZUME Tsutomu

  Faculty of Agriculture, Iwate University

• MASUDA Hiroshi

  Faculty of Agriculture, Iwate University

Bibliographic Information

Other Title

• ブタ精子の１０℃保存における精しょう濃度が精子の運動性に及ぼす影響
• ブタ精子の10℃保存における精漿濃度が精子の運動性に及ぼす影響
• ブタ セイシ ノ 10ドC ホゾン ニ オケル セイショウノウド ガ セイシ ノ ウンドウセイ ニ オヨボス エイキョウ

Abstract

This experiment was designed to clarify the optimum concentration of the seminal plasma in Modena extender for the liquid storage of boar semen at 10°C.<br>The sperm rich fraction was collected from 2 Landrace boars. About half volume of the semen was centrifuged to collect the seminal plasma. Washed spermatozoa were suspended to 1×10⁸ spermatozoa/ml with Modena extender that contained either 0, 1, 5, 15, 25, 50 or 75% seminal plasma. As a control the sperm rich fraction was diluted five fold with Modena extender. Each sperm suspension was stored anaerobicaly in 0.5ml microtube for 28 days at 10°C. The motility and progressive of spermatozoa were analyzed on the 1, 7, 14, 21 and 28 days of storage by Hamilton-Thorn Motility Analyzer. Each stored semen was diluted to 3×10⁷ spermatozoa/ml with Modena extender and incubated for 60 minutes at 38°C prior to analysis.<br>The motility and progressive of boar spermatozoa stored in Modena extender containing 5 to 25% seminal plasma were maintained better than those stored in Modena extender containing 0, 1, 50 and 75% of seminal plasma.<br>This result indicates that the optimum concentration of the seminal plasma in Modena extender is 5 to 25%. This state can be accomplished by a general method of diluting the sperm rich fraction in the range of 1:3 to 1:10 with an extender.

Journal

• Nihon Yoton Gakkaishi

  Nihon Yoton Gakkaishi 36 (1), 18-22, 1999

  The Japanese Society of Swine Science

Keywords

• seminal plasma
• liquid preservation
• dilution rate
• boar spermatozoa

Details

• CRID: 1390282679684143744
• NII Article ID: 10008285209
• NII Book ID: AN10202971
• DOI: 10.5938/youton.36.18
• ISSN: 1881655X
• ISSN: 0913882X
• ISSN: http://id.crossref.org/issn/13460706
• NDL BIB ID: 4682716
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030590784
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4682716
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed