Activation of Transepithelial Ion Transport by Secretin in Human Intestinal Caco-2 Cells

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Secretin stimulates bicarbonate secretion from pancreatic duct cells, but what influence secretin exerts on intestinal tissues remains to be clarified. The aim of this study is to examine effects of secretin on ion transport in intestinal epithelial Caco-2 cells. We mounted monolayers of Caco-2 cells grown on permeable supports for 21–28 d in a Ussing chamber and measured short-circuit currents (I<sub>sc</sub>). Addition of secretin (5–100 nM) to the basolateral solution dose-dependently induced biphasic increases of I<sub>sc</sub> (transient and sustained phase). Dibutyryl cyclic AMP (200 μM), forskolin (10 μM), and 3-isobutyl-1-methylxanthine (IBMX, 1 mM) also induced I<sub>sc</sub> responses similar to the administration of secretin. Addition of 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, 100 μM) or benzamil (100 μM) to the apical solution markedly reduced the secretin-induced I<sub>sc</sub> increase in the transient phase. A selective antagonist of cAMP-dependent protein kinase (PKA), N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89, 1 μM), and a membrane permeable Ca<sup>2+</sup> chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA/AM, 10 μM) reduced the secretin-induced I<sub>sc</sub>. Basolateral addition of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS, 1 mM) suppressed the sustained phase I<sub>sc</sub> increase. Secretin also induced alkalinization of the apical solution (ΔpH, 0.053 ± 0.013). The alkalinization did not occur when DIDS (1 mM) was added to the basolateral solution or Na<sup>+</sup> was removed from the solutions. Taken together, our observations suggest: (1) secretin stimulates a benzamil-sensitive Na<sup>+</sup> influx and an NPPB-sensitive Cl<sup>−</sup> efflux across the apical membrane through PKA-dependent and Ca<sup>2+</sup>-sensitive pathways; and (2) secretin also induces alkalinization of the apical solution through the activation of a DIDS-sensitive Na<sup>+</sup>-HCO<sub>3</sub><sup>−</sup> cotransport in the basolateral membrane of Caco-2 cells.<br>

収録刊行物

  • The Japanese journal of physiology

    The Japanese journal of physiology 50(2), 215-225, 2000-04

    PHYSIOLOGICAL SOCIETY OF JAPAN

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各種コード

  • NII論文ID(NAID)
    10008290962
  • NII書誌ID(NCID)
    AA00691224
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0021521X
  • NDL 記事登録ID
    5413503
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z53-D40
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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