Receptor-Mediated Facilitation of Cell Volume Regulation by Swelling-Induced ATP Release in Human Epithelial Cells

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Osmotic swelling induces the release of intracellular ATP in a number of cell types. In the immediate vicinity of the cell surface, released ATP has been shown to reach a concentration high enough to stimulate P2-purinergic receptors in a human epithelial cell line, Intestine 407. The role of released ATP in the regulatory volume decrease (RVD) after cell swelling was thus studied in Intestine 407 cells. The RVD was suppressed by an ATP hydrolyzing enzyme, apyrase, or by a purinergic receptor antagonist, suramin. Extracellular application of ATP accelerated the RVD rate in a concentration-dependent manner. An increase in the cytosolic free-Ca<sup>2+</sup> concentration was induced by a hypotonic challenge, and the swelling-induced Ca<sup>2+</sup> response was partially suppressed by apyrase or suramin. A rise in cytosolic Ca<sup>2+</sup> was also induced by extracellular application of ATP or UTP, but not ADP, 2-methylthio-ATP or α,β-methylene ATP. The ATP-induced Ca<sup>2+</sup> response was blocked by suramin. Therefore, it is concluded that RVD is facilitated by ATP, which is released upon cell swelling, by augmenting intracellular Ca<sup>2+</sup> rise via the stimulation of purinergic (P2Y<sub>2</sub>) receptors in the human epithelial cell.<br>

収録刊行物

  • The Japanese journal of physiology

    The Japanese journal of physiology 50(2), 235-241, 2000-04

    PHYSIOLOGICAL SOCIETY OF JAPAN

参考文献:  31件中 1-31件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10008291036
  • NII書誌ID(NCID)
    AA00691224
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0021521X
  • NDL 記事登録ID
    5413519
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z53-D40
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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