Mobilization of Intracellular Ca〔2+〕 by Thromboxane A2 Does Not Affect Ca〔2+〕-Activated K〔+〕 Channels in Rat Colonic Crypt Cells

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  • Mobilization of Intracellular Ca2+ by Thromboxane A2 Does Not Affect Ca2+-Activated K+ Channels in Rat Colonic Crypt Cells.

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The effects of 9,11-epithio-11,12-methano-thromboxane A2 (STA2), a stable thromboxane A2 analogue, and carbachol on colonic Ca2+-activated K+ channels were studied. In indo-1-loaded single cells in isolated rat colonic crypts, both STA2 (0.1 μm) and carbachol (10 μm) transiently increased intracellular free Ca2+ concentration ([Ca2+]i) by 136 and 155 nm, respectively. In whole-cell current-clamp experiments of the colonic crypt cells with Cl−-free solutions, carbachol (10 μm) hyperpolarized the cell by 19.7 mV, while STA2 (0.1 μm) did not affect the membrane potential. In the isolated colonic mucosa that was permeabilized mucosally by a monovalent ionophore nystatin in the presence of a serosally directed K+ gradient, carbachol (10 μm) transiently elicited K+ current, but STA2 (0.1 μm) did not. These results indicate that STA2 elevates [Ca2+]i in rat colonic crypt cells but does not activate basolateral Ca2+-activated K+ channels.<br>

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