Characteristics of Proton NMR T_2 Relaxation of Water in the Normal and Regenerating Tendon

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The molecular behavior of water in normal and regenerating tendons was analyzed using the transverse relaxation time (T<sub>2</sub>) measured by spin-echo proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy at 2.34 T (25°C). A section of the Achilles tendon was dissected from an anesthetized Japanese white rabbit, and its longitudinal axis was oriented at 0, 35, 54.7, 75, and 90° to the static magnetic field. In the normal tendon, the T<sub>2</sub> relaxation of water presented biexponential relaxation and anisotropy in both the long T<sub>2</sub> (5.41 to 6.21 ms) and short T<sub>2</sub> (0.41 to 1.43 ms) components, in which the greatest values were obtained at 54.7°. However, the range of the anisotropy was much narrower than we expected from the <sup>1</sup>H dipolar interaction of water bound to the collagen fibers in the tendon. The apparent fractions of water proton density also varied with orientation: the fraction of the longer T<sub>2</sub> components was at its maximum at 54.7°. These results suggest that a simple two-compartment model could not be applicable to orientational dependency of the T<sub>2</sub> value of the tendon, and the well ordered water in the short T<sub>2</sub> relaxation component may show an elongated T<sub>2</sub> relaxation time that falls in the range of the long T<sub>2</sub> relaxation component at 54.7°. This hypothesis can explain both the narrower range of the T<sub>2</sub> relaxation time and the orientational dependency on the apparent fraction of <sup>1</sup>H density. Regenerating processes of the Achilles tendon were followed for 18 weeks by analyzing the T<sub>2</sub> relaxation time. There is only a long T<sub>2</sub> relaxation time component (21.8 to 28.0 ms) up to 3 weeks after transection. Biexponential relaxation is revealed at 6 weeks and thereafter, whereby (i) the T<sub>2</sub> relaxation times become shorter, (ii) there is anisotropy in the short and long T<sub>2</sub> values, and (iii) the orientational dependency of the apparent fraction of water proton density becomes evident with maturation of the regenerating tendon. From these results, the <sup>1</sup>H T<sub>2</sub> relaxation time of water might be used to monitor the healing process of collagen structures of the tendon non-invasively.<br>

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  • The Japanese journal of physiology

    The Japanese journal of physiology 50(6), 569-576, 2000-12-01

    PHYSIOLOGICAL SOCIETY OF JAPAN

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各種コード

  • NII論文ID(NAID)
    10008292344
  • NII書誌ID(NCID)
    AA00691224
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0021521X
  • NDL 記事登録ID
    5706276
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z53-D40
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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