Is Rabbit CLCA1 Related to the Basolateral Ca〔2+〕-Dependent Cl〔-〕 Channel of Gastric Parietal Cells?

• Sakai Hideki

  Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Ukai Masashi

• Ikari Akira

• Asano Shinji

• Takeguchi Noriaki

書誌事項

タイトル別名

• Is Rabbit CLCA1 Related to the Basolateral Ca2+-Dependent Cl- Channel of Gastric Parietal Cells?

抄録

An expression of mRNA coding the calcium-activated Cl⁻ channel-1 (CLCA1) in rabbit gastric parietal cells was examined to verify the possibility that the CLCA1 mediates housekeeping Cl⁻ channels in the basolateral membrane. In whole-cell voltage-clamp experiments of rabbit parietal cells, A23187 (2 μM), a Ca²⁺ ionophore, activated the basolateral Cl⁻ channels. The partial cDNA fragment of rabbit CLCA1 could be amplified from the total RNA of tracheal epithelium. A Northern blot analysis showed that rabbit CLCA1 mRNA of 3.4 kb is highly expressed in the tracheal epithelium, but not in the gastric parietal cells. Even in a more sensitive detection of rabbit CLCA1 mRNA by RT-PCR, no signal could be observed in the gastric parietal cells. These results suggest that the CLCA1 protein may not be a subunit of the housekeeping Ca²⁺-dependent Cl⁻ channel in the basolateral membrane of rabbit gastric parietal cells.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 51 (1), 121-125, 2001

  一般社団法人　日本生理学会

キーワード

• stomach
• Cl⁻ channel
• CLCA

詳細情報

• CRID: 1390282680017737856
• NII論文ID: 130004435757
• NII論文ID: 10008293114
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.51.121
• COI: 1:CAS:528:DC%2BD3MXivVGktrs%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 5739253
• PubMed: 11282004
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5739253
• Web Site: https://search.jamas.or.jp/link/ui/2001187845
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可