Tyrosine Kinase-Independent Extracellular Action of Genistein on the CFTR Cl^- Channel in Guinea Pig Ventricular Myocytes and CFTR-Transfected Mouse Fibroblasts

この論文にアクセスする

この論文をさがす

著者

抄録

The effects of genistein, a protein tyrosine kinase inhibitor, on the cystic fibrosis transmembrane conductance regulator (CFTR) Cl<SUP>-</SUP> channel were studied in guinea pig ventricular myocytes and in NIH3T3 mouse fibroblasts stably transfected with CFTR cDNA by the whole-cell patch-clamp technique. Genistein did not activate whole-cell Cl<SUP>-</SUP> currents when applied to the intracellular (pipette) solution. In contrast, when applied to the extracellular solution, genistein alone promptly activated the Cl<SUP>-</SUP> current in a fully reversible manner. Also, extracellular genistein reversibly potentiated the forskolin-activated Cl<SUP>-</SUP> current. However, both basal and forskolin-activated Cl<SUP>-</SUP> currents were not affected by other protein tyrosine kinase inhibitors, including herbimycin A, lavendustin A, tyrphostin 21, tyrphostin 47, and tyrphostin 51. A nonspecific inhibitor of protein phosphatases, orthovanadate, had no effect on the genistein-induced activation of CFTR. Pretreatment with a protein kinase inhibitor, either H-89 or H-7, or with an adenylate cyclase inhibitor, SQ 22536, also had no effect on the genistein-induced response. Thus, it is concluded that genistein alone activates CFTR by a protein tyrosine kinase-independent and protein phosphatase-independent mechanism from the extracellular side, but not from the intracellular side.

収録刊行物

  • The Japanese journal of physiology

    The Japanese journal of physiology 48(5), 389-396, 1998-10

    PHYSIOLOGICAL SOCIETY OF JAPAN

参考文献:  30件中 1-30件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008305487
  • NII書誌ID(NCID)
    AA00691224
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0021521X
  • NDL 記事登録ID
    4615132
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z53-D40
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ