結核菌薬剤感受性試験に関する小川培地を用いた比率法と微量液体希釈法,BrothMIC MTBの判定互換性 INTERPRETIVE COMPATIBILITY OF ANTIMYCOBACTERIAL SUSCEPTIBILITY FOR MYCOBACTERIUM TUBERCULOSIS DETERMINED BY PROPORTION TEST METHOD ON EGG-BASED OGAWA MEDIA AND BROTH MICRODILUTION TEST, BrothMIC MTB

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著者

    • 比嘉 美也子 HIGA Miyako
    • 琉球大学医学部附属病院検査部 Clinical Laboratories, University Hospital School of Medicine, University of the Ryukyus
    • 仲宗根 勇 NAKASONE Isamu
    • 琉球大学医学部附属病院検査部 Clinical Laboratories, University Hospital School of Medicine, University of the Ryukyus
    • 宮城 知佳 MIYAGI Chika
    • 琉球大学医学部臨床検査医学講座 Department of Laboratory Medicine, Faculty of Medicine, University of the Ryukyus

抄録

日本結核病学会が提案する結核菌薬剤感受性試験, 小川培地での比率法を, 微量液体希釈法を原理とするBrothMIC MTB-1 (極東製薬工業, 東京) と比較評価した. 評価ではstreptomycin, ethambutol, kanamycin, isoniazid, dfampicinを試験対象にした. これらの薬剤に対し, 3株の標準株を反復試験して再現性を評価したが, いずれの試験方法も高い再現性を示した. BrothMIC MTB法で測定された最小発育阻止濃度 (MIC) は, すべて±1管, 31092に分布したが, 計11件のMICが判定保留の判定となった. 他方, 小川培地での比率法ではすべての試験結果が期待される判定となった. しかし48件の試験のうち, 3件が菌発育対照培地での不十分な菌発育から, 判定不能となった. 127株の臨床材料由来の結核菌が試験され, 判定一致率は89~90%に評価された. BrothMIC MTB法で7.1~9.4%が判定保留に, まったく乖離した判定は0.8-3.1%に観察された.<BR>結論として, これら2つの試験方法には高い再現性と互換性が示された. それぞれの試験方法のもつ長所と短所を考察した.

The antimycobacterial susceptibility test method newly proposed by the Japanese Society for Tuberculosis, a proportion method on egg-based Ogawa media, was evaluated in comparison with microdilution test for Mycobacterium tuberculosis complex, BrothMIC MTB-1 (Kyokuto Pharmaceutical Inc., Tokyo). In the evaluation, five antimicrobial agents, streptomycin, ethambutol, kanamycin, isoniazid and rifampicin were included. Through repeated testings of the three reference strains against five antimicrobial agents, both test methods were found to be highly precise. All the minimum inhibitory concentrations (MICs) determined by BrothMIC MTB fell within 3 log2 dilutions, however a total of 11 MICs resulted in indeterminate (I) interpretations. Whereas, all the test results by a proportion method on Ogawa media were comparable to the expected interpretations. However, three of 48 testings resulted in undeterminable interpretations due to insufficient growth on the growth control media. A total of 127 clinical isolates of M tuberculosis complex were tested by both methods, and 89 to 90 % of the test results were comparable with each other in category interpretations. However, 7.1 to 9.4 % of MICs determined by BrothMIC MTB resulted in indeterminate (I), and 0.8 to 3.1% of discrepant interpretations were observed.<BR>In conclusion, both test methods were highly precise and comparable in determining antimycobacterial susceptibility for M tuberculosis complex. Several advantages and disadvantages in each test method were discussed.

収録刊行物

  • 結核

    結核 77(2), 61-66, 2002-02-15

    JAPANESE SOCIETY FOR TUBERCULOSIS

参考文献:  10件中 1-10件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10008385118
  • NII書誌ID(NCID)
    AN00073442
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00229776
  • NDL 記事登録ID
    026778795
  • NDL 請求記号
    Z19-133
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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