Characteristics of ATP-Induced Current Through P2X_7 Receptor in NG108-15 Cells : Unique Antagonist Sensitivity and Lack of Pore Formation

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ATP activates the mouse P2X<sub>7</sub> receptor and induces a nonselective-cation current in NG108-15 cells. We investigated the effects of five receptor antagonists on the ATP-induced nonselective-cation current through P2X<sub>7</sub> receptor (I<sub>NS · P2X7</sub>) in NG108-15 cells. Nonselective P2 receptor antagonists, RB-2, PPADS and suramin inhibited the I<sub>NS · P2X7</sub> with IC<sub>50</sub> values of 4.3, 53 and 40 μM, respectively. However, KN-04, which is a potent antagonist of human P2X<sub>7</sub> receptors but is not that of rat P2X<sub>7</sub> receptors, had only a weak blocking effect. Furthermore, oxidized-ATP (300 μM), an antagonist of the P2X<sub>7</sub> receptor-mediated pore-formation, did not affect the I<sub>NS · P2X7</sub>. Prolonged ATP application did not increase the membrane permeability to large molecules, <i>N</i>-methyl-<sc><font size = -2>D</font></sc>-glucamine or Yo-Pro-1, indicating that pore-formation was not promoted by the P2X<sub>7</sub> receptor activation in NG108-15 cells. These results suggest that antagonist sensitivities and pore-forming properties of the P2X<sub>7</sub> receptors in NG108-15 cells are different from those of other cells types.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 88(4), 428-435, 2002-04-01

    The Japanese Pharmacological Society

参考文献:  41件中 1-41件 を表示

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各種コード

  • NII論文ID(NAID)
    10008415187
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    6263262
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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