Characteristics of ATP-Induced Current Through P2X7 Receptor in NG108-15 Cells: Unique Antagonist Sensitivity and Lack of Pore Formation.
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- Watano Tomokazu
- Department of Pharmacology, Fukushima Medical University School of Medicine
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- Matsuoka Isao
- Department of Pharmacology, Fukushima Medical University School of Medicine
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- Kimura Junko
- Department of Pharmacology, Fukushima Medical University School of Medicine
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ATP activates the mouse P2X7 receptor and induces a nonselective-cation current in NG108-15 cells. We investigated the effects of five receptor antagonists on the ATP-induced nonselective-cation current through P2X7 receptor (INS · P2X7) in NG108-15 cells. Nonselective P2 receptor antagonists, RB-2, PPADS and suramin inhibited the INS · P2X7 with IC50 values of 4.3, 53 and 40 μM, respectively. However, KN-04, which is a potent antagonist of human P2X7 receptors but is not that of rat P2X7 receptors, had only a weak blocking effect. Furthermore, oxidized-ATP (300 μM), an antagonist of the P2X7 receptor-mediated pore-formation, did not affect the INS · P2X7. Prolonged ATP application did not increase the membrane permeability to large molecules, N-methyl-<sc>D</sc>-glucamine or Yo-Pro-1, indicating that pore-formation was not promoted by the P2X7 receptor activation in NG108-15 cells. These results suggest that antagonist sensitivities and pore-forming properties of the P2X7 receptors in NG108-15 cells are different from those of other cells types.
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 88 (4), 428-435, 2002
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204285787008
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- NII論文ID
- 10008415187
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- NII書誌ID
- AA00691188
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- COI
- 1:STN:280:DC%2BD38zgsFGmtQ%3D%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 6263262
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- PubMed
- 12046986
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可