Differentiating the Biosynthesis of Pseudomonic Acids A and B.

• MANTLE PETER G.

  Department of Biochemistry, Imperial College of Science, Technology and Medicine

• LANGEN MATTHIJS DE

  Department of Biochemistry, Imperial College of Science, Technology and Medicine

• TEO VEI KIM

  Department of Biochemistry, Imperial College of Science, Technology and Medicine

抄録

Pseudomonic acid A (1) has been the dominant commercial pseudomonate antibiotic produced by Pseudomonas fluorescens. In specific shaken flask conditions initial fermentation accumulation of 1 is followed by preferential accumulation of the 8-hydroxy derivative, pseudomonic acid B (2). Biosynthetic probing with a pulse of [1-¹⁴C] acetate or L-[methyl-¹⁴C] methionine at early, mid and late stages of the fermentation gave relative patterns of radioactivity in 1 and 2 that are inconsistent with an assumption that 2 arises by oxidation of 1, or that 1 is formed by reduction of 2. Since [methyl-¹⁴C] methionine only labels carbons in the 12-carbon part of the pseudomonate molecule that is thought to be an early biosynthetic moiety, the evidence from radiolabelling experiments implies that preferential early oxidation of this biosynthetic intermediate causes the pathway diversion to accumulate 2 instead of 1.

収録刊行物

• The Journal of Antibiotics

  The Journal of Antibiotics 54 (2), 166-174, 2001

  公益財団法人 日本感染症医薬品協会

キーワード

• Drug Discovery
• Pharmacology

詳細情報

• CRID: 1390001204150283648
• NII論文ID: 10008469798
• NII書誌ID: AA0069330X
• DOI: 10.7164/antibiotics.54.166
• COI: 1:CAS:528:DC%2BD3MXhsFyntrk%3D
• ISSN: 18811469
• ISSN: 00218820
• PubMed: 11302490
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可