Tyropeptins A and B, New Proteasome Inhibitors Produced by Kitasatospora sp. MK993-dF2. I. Taxonomy, Isolation, Physico-chemical Properties and Biological Activities.
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- MOMOSE ISAO
- Institute of Microbial Chemistry
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- SEKIZAWA RYUICHI
- Institute of Microbial Chemistry
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- HASHIZUME HIDEKI
- Institute of Microbial Chemistry
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- KINOSHITA NAOKO
- Institute of Microbial Chemistry
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- HOMMA YOSHIKO
- Institute of Microbial Chemistry
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- HAMADA MASA
- Institute of Microbial Chemistry
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- IINUMA HIRONOBU
- Institute of Microbial Chemistry
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- TAKEUCHI TOMIO
- Institute of Microbial Chemistry
Bibliographic Information
- Other Title
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- I. Taxonomy, Isolation, Physico-chemical Properties and Biological Activities
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Abstract
Tyropeptins A and B, new proteasome inhibitors, were isolated from the culture broth of Kitasatospora sp. MK993-dF2. They were purified using ethyl acetate extraction, silica gel column chromatography, Sephadex LH-20 column chromatography and HPLC. Tyropeptin A inhibited the chymotrypsin-like (ChT-L) and trypsin-like (T-L) activities of 20S proteasome with IC50 values of 0.1μg/ml and 1.5μg/ml respectively, but did not inhibit the peptidylglutamyl-peptide hydrolyzing (PGPH) activity of 20S proteasome at a concentration of 100μg/ml. The inhibitory activities of tyropeptin A were about two times as strong as that of tyropeptin B. Taxonomy of the producing strain is also described.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 54 (12), 997-1003, 2001
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
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Details 詳細情報について
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- CRID
- 1390001204151009792
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- NII Article ID
- 130003503832
- 10008471494
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- NII Book ID
- AA0069330X
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- COI
- 1:CAS:528:DC%2BD38XhtF2msA%3D%3D
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- ISSN
- 18811469
- 00218820
- http://id.crossref.org/issn/00218820
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- PubMed
- 11858671
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed