塩酸ニフェカラントの心室頻拍リエントリー回路への作用 Effects of Nifekalant on reentrant circuit of sustained ventricular tachycardia

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著者

    • 鷲塚 隆 WASHIZUKA Takashi
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 池主 雅臣 CHINUSHI Masaomi
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 杉浦 広隆 SUGIURA Hirotaka
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 小村 悟 KOMURA Satoru
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 渡部 裕 WATANABE Hiroshi
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 保坂 幸男 HOSAKA Yukio
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 田辺 靖貴 TANABE Yasutaka
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 山浦 正幸 YAMAURA Masayuki
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 古嶋 博司 FURUSHIMA Hiroshi
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 阿部 晃 ABE Akira
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital
    • 相澤 義房 AIZAWA Yoshifusa
    • 新潟大学大学院医歯学総合研究科器官制御医学講座循環器分野 First Department of Internal Medicine, Niigata University Hospital

抄録

DNA nanotechnology-based nanosystems and macrosystems have attracted much attention in the biomedical research field. The nature of DNA endows these systems with biodegradable, biocompatible, and immunomodulatory properties. Here, we present an injectable hydrogel system that consists only of chemically synthesized short DNA strands, water, and salts. Several preparations of polypod-like structured DNA, or polypodna, were designed, including tri-, tetra-, penta- and hexapodna, as the building blocks of self-gelling DNA hydrogel. Under physiological conditions, properly designed polypodna preparations formed a hydrogel. The analysis of the modulus data of the hydrogel consisting of two sets of hexapodna preparations showed that this injectable hydrogel was reorganized at a time scale of 0.25s. Then, DNA hydrogel containing unmethylated cytosine-phosphate-guanine (CpG) dinucleotides was used to stimulate innate immunity through Toll-like receptor 9, the receptor for CpG DNA. Gel formation significantly increased the activity of immunostimulatory CpG DNA, retarded the clearance after intradermal injection into mice, and increased the immune responses to ovalbumin (OVA) incorporated into the hydrogel as a model antigen. OVA/CpG DNA hydrogel induced much less local or systemic adverse reactions than OVA injected with complete Freund's adjuvant or alum. GpC DNA hydrogel containing no CpG sequences was less effective, indicating the importance of immunomodulation by CpG DNA hydrogel. Thus, we have created an efficient system for sustained delivery of antigens or other bioactive compounds.

収録刊行物

  • 不整脈 = Journal of arrhythmia

    不整脈 = Journal of arrhythmia 18(1), 25-32, 2002-01-25

    Elsevier B.V.

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