Changes in the Function of Lymphocyte Blast Formation with Aging Estimated by Ethidium Bromide Fluorescence Assay.

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  • 加齢およびアルツハイマー病によるリンパ球幼若化能の変化　蛍光測光法による検討
  • カレイ オヨビ アルツハイマービョウ ニ ヨル リンパキュウ ヨウジャクカノウ
  • 蛍光測光法による検討

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Abstract

Modification of DNA with aging has been proposed as a mechanism of cellular senescence. To test this hypothesis, we measured fluorescence of the DNA-Ethidium bromide (EB) complex in human peripheral lymphocytes.<br>Lymphocytes were incubated in a medium containing phytohemagglutinin (PHA-P). EB was linked to lymphoblast-DNA.<br>Healthy adults of three age groups were examined: 40-49 (N=14), 50-59 (N=16), 60-69 (N=8). Moreover, we studied lymphocytes from 17 patients (47-74 years old) with probable Alzheimer's disease.<br>An aged-related linear decrease in fluorescence intensity was found in healthy controls (r=0.135, P<0.05), and for Alzheimer's disease patients (r=0.443, P<0.10). The regression equation are:<br>Y=-0.0405X+7.164 (Healthy Contorols)<br>Y=-0.121X+11.258 (Alzheimer's disease patients)<br>where X and Y are age and fluorescence, respectively.<br>These results indicate that the analysis of DNA-EB fluorescence in lymphocytes may be useful in the study of changes associated with aging, and also in the evaluation of the clinical diagnosis of Alzheimer's disease.

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