ユーグレナ（Ｅｕｇｌｅｎａ　ｇｒａｃｉｌｉｓ　Ｚ）加水分解物の降圧作用

DOI Web Site 参考文献28件

書誌事項

タイトル別名
  • Hypotensive Effect of Euglena (Euglena gracilis Z) Pepsin Hydrolysate.
  • ユーグレナ Euglena gracilis Z カスイ ブンカイブツ ノ コ

この論文をさがす

抄録

Euglena hydrolysate prepared from deffatted Euglena dry cells (Euglena glacilis Z) by treatment with pepsin was tested for inhibitory activity against angiotensin I converting enzyme (ACE) in serum and vascular tissues and for hypotensive effects in stroke-prone spontaneously hypertensive rats (SHRSP). The effect of captopril, an ACE inhibitor, was also studied in comparison. When Euglena hydrolysate was fractionated by gel filtration on Sephadex G-25 Fine, inhibitory activity was found in many Fractions, but Fraction D (molecular weight of the principle being approximately 2, 000) showed the strongest activity. The IC50 value of Fraction D to ACE (25mU/ml) from rabbit lung was 12μg protein/ml. The inhibitory activity of Fraction D was retained even after in vitro digestion by trypsin and chymotrypsin. When Fraction D wasorally administered to SHR and SHRSP for 8 days at a dose of 8mg protein/rat/day, pressure fell by about 30 and 20mmHg, respectively, from the original level. The serum ACE activity of SHRSP pon administration of Fraction D showed no difference from that of controls given 0.9% saline. In the SHRSP given captopril, the serum ACE activity was enhanced. The thoracic aorta ACE activity was lowered to a greater degree (p<0.05) in the Fraction D group and captopril group than in the control group. In the mesenteric artery, the ACE activity of Fraction D group was somewhat lower than that in the control group. The ACE activities of the thoracic aorta and mesenteric artery in the captopril group were significantly lower (p<0.05) than those in the controls. In perfused mesenteric artery isolated from SHRSP, Fraction D (500μg protein/ml) inhibited the contraction caused by angiotensin I (1μg/ml), as did captopril. These results suggest that the hypotensive effect of Fraction D in SHRSP is due to its inhibitive action on vascular tissue ACE activity.

収録刊行物

参考文献 (28)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ