Expression of PCNA, Basic Fibroblast Growth Factor, FGF-receptor and Vascular Endothelial Growth Factor in Adenomas and Carcinomas of Human Colon.

• Shiraishi Atsushi

  Department of Pathology, Nippon Medical School Second Department of Surgery, Nippon Medical School

• Ishiwata Toshiyuki

  Department of Pathology, Nippon Medical School

• Shoji Tasuku

  Second Department of Surgery, Nippon Medical School

• Asano Goro

  Department of Pathology, Nippon Medical School

Abstract

This study was designed to clarify the role of growth factors and vascular proliferation in the growth of colon tumor. On immunohistochemical observation, the ratios of positive cells for PCNA, bFGF and VEGF in carcinomas and adenomas were higher than in non-neoplastic mucosa. On reverse transcriptasepolymerase chain reaction (RT-PCR) study, the expression of bFGF mRNA, FGF-receptor (FGFR) mRNA and VEGF mRNA was confirmed in all cases including non-neoplastic tissues, adenomas and carcinomas. On in situ hybridization, bFGF mRNA was expressed in carcinoma cells, vascular cells and fibroblastic cells, and the expression ratio of bFGF mRNA in carcinomas was higher than in non-carcinomatous mucosa. VEGF mRNA was expressed in carcinoma cells, medial smooth muscle cells and fibroblastic cells. These results suggest that bFGF and VEGF may contribute to tumor growth in association with neovascularization.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 28 (1), 21-29, 1995

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• Human colon carcinoma
• Proliferating cell nuclear antigen
• Basic fibroblast growth factor
• Vascular endothelial growth factor
• <i>In situ</i> hybridization

Details

• CRID: 1390282679838234112
• NII Article ID: 10008603387
• NII Book ID: AA00508022
• DOI: 10.1267/ahc.28.21
• ISSN: 13475800
• ISSN: 00445991
• ISSN: http://id.crossref.org/issn/00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/28/1/28_1_21/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996027199
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed