Detection of a Helicobacter pylori Gene Marker in Gastric Biopsy Samples by Non-radioactive in situ Hybridization

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The presence of <I>H. pylori</I> was examined by non-radioactive <I>in situ</I> hybridization using a digoxigenin-labelled DNA probe and a synthetic <I>H. pylori</I> urease gene-specific thymine-thymine dimerized oligonucleotide probe. <I>H. pylori</I> was detected in 20/33 gastric biopsy specimens, and was localized in gastric epithelia and neutrophils. No nonspecific DNA signal was detected in colonic mucosa, esophageal mucosa, skin and liver specimens. The DNA probe did not crosshybridize with DNA from other bacteria. As this method is specific and not time-consuming, it may be useful for revealing the location of <I>H. pylori</I>.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 28(3), 263-267, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

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各種コード

  • NII論文ID(NAID)
    10008603874
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  J-STAGE 
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