Simple and Rapid Method for Detection of Apoptotic Cells by Flow Cytometry

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We have developed a simple and rapid method for detection of apoptotic cells by flow cytometry -a modified nick end-labeling method. DNA fragments <I>in situ</I> associated with apoptosis are detected by the nick-end labeling method (TUNEL method) in our new method. This method is well suited for easy identification of cell types and for <I>in situ</I> measurement of minute quantities of apoptotic cells. We present a modified method for nick-end labeling by flow cytometry (NELF) to detect apoptosis of both cultured cells and blood cells and also to analyze DNA fragments quantitatively and at the single-cell level. We modified the NELF method to simplify procedures and to save time. The protocol consists of 1) fixation with cold acetone, 2) transfer of biotiny lated nucleotide to the 3′-OH end with terminal deoxynucleotidyl transferase, and 3) incubation with streptoavidin-conjugated phycoerythrin to biotinylated nucleotide. This method may be useful in two-color analysis with a wide variety of antibodies.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 28(3), 277-280, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

参考文献:  19件中 1-19件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10008603936
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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