Aminopeptidases and Dipeptidyl Peptidases Activities in Chiclken Bone Tissue

この論文にアクセスする

この論文をさがす

著者

抄録

There have been very few papers reporting on the localizations of aminopeptidases and dipeptidyl peptidases activities in bone tissue, although these peptidases seem to have important roles in bone resorption and osteoid degradation. The present study demonstrates these peptidases in fixed and decalcified tibial metaphyses of 3-week-old chickens using the azo-dye methods at light microscopic level. As substrates, amino acid derivatives of 4-methoxy-2-naphthylamine (MNA) were used: Leu- or Ala-MNA for aminopeptidase-M (AP-M, EC 3.4.11.2), Glu-MNA for aminopeptidase-A (AP-A, EC 3.4.11.7), Gly-Arg-MNA for dipeptidyl peptidase-I (DPP-I, EC 3.4.14.1), Lys-Ala-or Lys-Pro-MNA for dipeptidyl peptidase-II (DPP-II, EC 3.4.14.2), and Gly-Pro-MNA for dipeptidyl peptidase-IV (DPP-IV, EC 3.4.14.5). An AP-M in osteoclasts, and APA in osteoblasts and osteocytes were observed. These activities were sensitive to 10 mM of ethylenediaminetetraacetic acid (EDTA). A DPP-I was seen in osteoclasts, osteoblasts and osteocytes in the presence of mercaptoethylamine (MEA), however Gly-Arg-MNA hydrolyzing activity in the absence of MEA was restricted to only osteoclasts. DPP-II activity was present in osteoblasts and osteocytes, using Lys-Pro-MNA as a substrate. There was no Lys-Ala-MNA hydrolyzing activity in bone cells. The present study failed to demonstrate DPP-IV activity in either 4% paraformaldehyde- or 2.5% glutaraldehyde-fixed samples, although it was observed in the glomerulus and proximal convoluted tubule cells of the rat kidney fixed with glutaraldehyde. The present study suggests that AP-M and DPP-I in osteoclasts, and AP-A, DPP-I and DPP-II in osteoblasts seem to function in the bone remodeling process.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 28(3), 281-286, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

参考文献:  22件中 1-22件 を表示

各種コード

  • NII論文ID(NAID)
    10008603956
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  J-STAGE 
ページトップへ