Multi-color FISH: Application to the Simultaneous Detection of Chromosome Aberrations in Neuro-epithelial Tumor Cell Lines.

DOI Web Site 23 References
  • Hashimoto Naoya
    Departments of Hygiene, Kyoto Prefectural University of Medicine Departments of Neurosurgery, Kyoto Prefectural University of Medicine
  • Inazawa Johji
    Departments of Hygiene, Kyoto Prefectural University of Medicine
  • Matsumura Takafumi
    Departments of Pediatrics, Kyoto Prefectural University of Medicine
  • Ichikawa Daisuke
    Departments of Hygiene, Kyoto Prefectural University of Medicine
  • Ishino Shinsuke
    Departments of Hygiene, Kyoto Prefectural University of Medicine Departments of Neurosurgery, Kyoto Prefectural University of Medicine
  • Houri Takashi
    Departments of Neurosurgery, Kyoto Prefectural University of Medicine
  • Ueda Satoshi
    Departments of Neurosurgery, Kyoto Prefectural University of Medicine
  • Abe Tatsuo
    Departments of Hygiene, Kyoto Prefectural University of Medicine

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Abstract

Fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes allows cytogenetic information to be obtained rapidly and precisely from metaphase and interphase chromosomes. Multi-color FISH with two or more probes makes it feasible to delineate several chromosome aberrations simultaneously occurring in a nucleus. Here we present a simple and rapid method for multi-color FISH and its application to the cytogenetic investigation of tumor cells. In addition, the usefulness of this method is discussed.

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