Immunolocalization of Glutathione-Peroxidase (GSH-PO) and Bc1-2 in the Rat Ventral Prostate. Effects of Castration and Administration of Testosterone.
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- Murakoshi Masanori
- Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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- Ikeda Rie
- Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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- Tagawa Masashi
- Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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- Suzuki Minoru
- Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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- Takekoshi Susumu
- Department of Pathology, Tokai University School of Medicine
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- Watanabe Keiichi
- Department of Pathology, Tokai University School of Medicine
Bibliographic Information
- Other Title
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- Effects of Castration and Administration of Testosterone
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Abstract
Immunolocalization of glutathione-peroxidase (GSH-PO), apoptosis and bcl-2 protein in the rat ventral prostate was investigated under the presence or absence of androgen. Male Sprague-Dawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrificed two days after castration. In groups 3 and 4, rats were subcutaneously administered 1 mg/animal of testosterone-propionate daily for three or seven days after two days of castration. The intensity of GSH-PO staining in the glandular epithelial cells of the ventral prostate was remarkably decreased after castration (Group 2), and it was clearly recovered by testosterone-administration (Groups 3 and 4) to the castrated rats. The prostatic GSH-PO mRNA levels were diminished in the castrated rat ventral prostate but greatly increased by testosterone (Groups 3 and 4). Furthermore, castration (Group 2) induced apoptosis in the prostatic glandular epithelial cells and apoptosis was reduced by testosterone-administration (Groups 3 and 4) to the castrated rats. In groups 3 and 4, expression of bcl-2 protein was clearly detected in the glandular epithelial cells of the ventral prostate. These findings strongly suggest that expression of GSH-PO and bcl-2 protein in the glandular epithelial cells of the rat ventral prostate is considered to be testosterone-dependent.
Journal
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- ACTA HISTOCHEMICA ET CYTOCHEMICA
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ACTA HISTOCHEMICA ET CYTOCHEMICA 29 (4), 283-288, 1996
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
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Details 詳細情報について
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- CRID
- 1390282679839900672
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- NII Article ID
- 10008605682
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- NII Book ID
- AA00508022
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- COI
- 1:CAS:528:DyaK2sXisFGns7s%3D
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- ISSN
- 13475800
- 00445991
- http://id.crossref.org/issn/00445991
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed