Enzymatic Histochemical Localization of Aldehyde Oxidase in Rat Liver by the Tetrazolium Method.

• Moriwaki Yuji

  Third Department of Internal Medicine, Hyogo College of Medicine

• Yamamoto Tetsuya

  Third Department of Internal Medicine, Hyogo College of Medicine

• Yamakita Jun-ichi

  Third Department of Internal Medicine, Hyogo College of Medicine

• Takahashi Sumio

  Third Department of Internal Medicine, Hyogo College of Medicine

• Higashino Kazuya

  Third Department of Internal Medicine, Hyogo College of Medicine

Abstract

The enzymatic histochemical localization of aldehyde oxidase in the rat liver lobule was investigated by use of a tissue protectant, polyvinyl alcohol, with tetra-nitro BT as the final electron acceptor. This method demonstrated the presence of aldehyde oxidase activity in the cytoplasm of liver cells. Distribution of aldehyde oxidase activity in the liver was uneven, being seen mainly in the pericentral rather than the periportal area as with xanthine oxidase. The reaction was not inhibited by TEI-6720 but was markedly inhibited by benzamidine. Results were consistent with those of immunohistochemical localization studies of this enzyme.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 30 (1), 113-115, 1997

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• Aldehyde oxidase
• Enzyme-histochemistry
• Rat liver

Details

• CRID: 1390001204861976064
• NII Article ID: 10008606250
• NII Book ID: AA00508022
• DOI: 10.1267/ahc.30.113
• COI: 1:CAS:528:DyaK2sXjtV2iu7o%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/30/1/30_1_113/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997171261
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed