Cell Growth of Rat Lens Epithelium in Galactose-induced Cataracts

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Cell growth of rat lens epithelium during galactose-induced cataract formation was examined in the present study using fluorescence cytophotometry or proliferating cell nuclear antigen (PCNA) immunohistochemistry. Six-week old male Sprague-Dawley rats were divided into 4 dietary groups according to galactose supplementation of the diet: 5%, 10%, 15% and 25% galactose diet groups. Whole-mount preparations of rat lens epithelium were prepared. The nuclear content of epithelial cells was measured using fluorescence cytophotometry, and cells undergoing DNA synthesis (PCNA labeled cells) were detected using the PCNA immunohistochemical staining method. The results of fluorescence cytophotometry showed that cells containing tetraploid nuclei (4C cells) gradually increased in number. In the 5% galactose diet fed rats, the number of 4C cells peaked on the 9th day after the start of dietary galactose supplementation, the 7th day in the 10% galactose diet fed rats and on the 5th day in the 15% and 25% galactose diet fed rats. On each day that 4C cells peaked, a large number of PCNA labeled cells were observed in the central zone of the epithelial cell layer. These results suggest that growth stimulation of lens epithelial cells depends upon the concentration of galactose in the diet and precedes changes in lens fiber.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 30(3), 243-249, 1997-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

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各種コード

  • NII論文ID(NAID)
    10008606805
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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