Ki-67 Antigen Retrieval in Formalin- or Ethanol-fixed, Paraffin-embedded Tissues : An Enhancement Method for Immunohistochemical Staining with Autoclave Treatment

この論文にアクセスする

この論文をさがす

著者

    • TSUJI Yoshiro
    • Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine
    • HIRASAWA Yasusuke
    • Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine
    • SERRA Massimo
    • Department of Research Oncology, Rizzoli Orthopaedic Institute

抄録

We developed a new immunohistochemical method to visualize Ki-67 antigen in formalin-fixed, paraffin-embedded sections of bone tumors. The method uses autoclave pretreatment of sections immersed in citrate buffer before incubation with the MIB 1 antibody, which reacts with the Ki-67 gene product. MIB 1 antibody showed strong and discrete staining of cell nuclei in proliferating tumor cells. This method also retrieved antigen in paraffin-embedded sections of alcohol-fixed tissue. Therefore, tissues previously considered unsuitable for immunohistochemical analysis can be studied after autoclave treatment.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 30(3), 251-255, 1997-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

参考文献:  8件中 1-8件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10008606822
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
ページトップへ