Ultrastructure and Immunolocalization of Transforming Growth Factor-Beta in Chondrification of Murine Ligamentous Fibroblasts and Endochondral Calcification Induced by Recombinant Human Bone Morphogenetic Protein-2

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著者

    • HOSHI Kazuto
    • First Department of Oral Anatomy, Niigata University School of Dentistry
    • AMIZUKA Norio
    • First Department of Oral Anatomy, Niigata University School of Dentistry
    • KUROKAWA Takahide
    • Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo
    • OZAWA Hidehiro
    • First Department of Oral Anatomy, Niigata University School of Dentistry

抄録

In order to elucidate the causes of ossification in spinal ligaments, ultrastructural alteration and immunolocalization of transforming growth factor-β (TGF-β), as well as that of the TGF-βreceptor were examined during the chondrification of ligamentous fibroblasts, by inducing ossification in ligamenta flava of murine lumbar spines, employing recombinant human bone morphogenetic protein-2 (rhBMP-2). Normal ligamenta flava, consisting of flattened fibroblasts, showed no immunolocalization of TGF-βisoforms. From the second week following BMP-administration, cartilage gradually replaced the ligaments. Cells in the central portion of the ligament contained abundant Golgi apparatus as well as enlarged endoplasmic reticula. Proliferative or hypertrophic chondrocytes in this area showed immunoreactivity to TGF-β3 latency-associated peptide, while calcified hypertrophic chondrocytes accompanied by both matrix vesicleand collagen-calcification were noticed at the insertion site to spinal lamina, where there was no immunolocalization of this peptide. In addition, the TGF-βtype I receptor was localized on the proliferative and hypertrophic cells of the central portion. Therefore, as regards the pathological chondrification of ligamentous fibroblasts induced by exogenous BMP-2, TGF-βis speculated to promote matrix-formation and chondrocytic maturation, under the autocrine/paracrine system, in concert with exogenous BMP-2.

In order to elucidate the causes of ossification in spinal ligaments, ultrastructural alteration and immunolocalization of transforming growth factor-β (TGF-β), as well as that of the TGF-β receptor were examined during the chondrification of ligamentous fibroblasts, by inducing ossification in ligamenta flava of murine lumbar spines, employing recombinant human bone morphogenetic protein-2 (rhBMP-2). Normal ligamenta flava, consisting of flattened fibroblasts, showed no immunolocalization of TGF-β isoforms. From the second week following BMP-administration, cartilage gradually replaced the ligaments. Cells in the central portion of the ligament contained abundant Golgi apparatus as well as enlarged endoplasmic reticula. Proliferative or hypertrophic chondrocytes in this area showed immunoreactivity to TGF-β<sub>3</sub> latency-associated peptide, while calcified hypertrophic chondrocytes accompanied by both matrix vesicle- and collagen-calcification were noticed at the insertion site to spinal lamina, where there was no immunolocalization of this peptide. In addition, the TGF-β type I receptor was localized on the proliferative and hypertrophic cells of the central portion. Therefore, as regards the pathological chondrification of ligamentous fibroblasts induced by exogenous BMP-2, TGF-β is speculated to promote matrix-formation and chondrocytic maturation, under the autocrine/paracrine system, in concert with exogenous BMP-2.

収録刊行物

  • Acta histochemica et cytochemica

    Acta histochemica et cytochemica 30(4), 371-379, 1997-08-01

    日本組織細胞化学会

参考文献:  21件中 1-21件 を表示

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各種コード

  • NII論文ID(NAID)
    10008607167
  • NII書誌ID(NCID)
    AA00508022
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00445991
  • データ提供元
    CJP書誌  CJP引用  IR  J-STAGE 
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