Expression of Cystatin C in Human Histiocytic Lymphoma, U-937, Cells.

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  • Barka Tibor
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine of The City University of New York Department of Pathology, Mount Sinai School of Medicine of The City University of New York
  • Noen Hendrika van der
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine of The City University of New York

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The established human histiocytic lymphoma U-937 cell line is of monocytic origin. U-937 cells can be induced by tumor promoters, Vitamin D3 and retinoic acid to differentiate into macrophage-like cells. U-937 cells express the gene for the cysteine proteinase inhibitor cystatin C. This was established by Northern blot hybridizations, reverse transcriptase-polymerase chain reaction and Western blots of partially purified cystatins from extracts of U-937 cells. Induction of macrophage-like differentiation by the tumor promoters phorbol-12-myristate-13-acetate or 12-O-tetradecanoylphorbol-13-acetate, or with Vitamin D3, or retinoic acid reduced the steady-state level of cystatin C mRNA in U-937 cells, suggesting that the expression of the cystatin C gene varies with the differentiation state. U-937 cells secrete cystatin C. This secretion was not affected significantly by the calcium ionophore A23187, PMA, or dibutyryl-adenosine 3′: 5′-cyclic monophosphate. Since macrophages play pivotal roles in immune and inflammatory reactions involving intra- and extra-cellular proteolysis, cystatin C produced and secreted by these cells probably has a modulatory function on the action of cysteine proteinases produced or released locally.

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